Steven N. Hart, Ph.D.

Ph.D. Student (2006-2011)

Current position: Associate Professor of Biomedical Informatics at Mayo College of Medicine

Education

Professional Appointments

Professional Societies

Honors and Awards

Publications

Hart SN, Li Y, Nakamoto K, Wesselman C, and Zhong XB. Novel SNPs in cytochrome P450 oxidoreductase. Drug Metabolism and Pharmacokinetics, 2007, 22(4):322-326. PMID:17827787.

Hart SN, Wang S, Nakamoto K, Wesselman C, Li Y, and Zhong XB. Genetic polymorphisms in cytochrome P450 oxidoreductase influence microsomal P450-catelyzed drug metabolism. Pharmacogenetics and Genomics, 2008, 18(1):11-24. PMID:18216718.

Hart SN and Zhong XB. P450 oxidoreductase: genetic polymorphisms and implications to drug metabolism and toxicity. Expert Opinion on Drug Metabolism and Toxicology, 2008, 4(4):439-452. Review. PMID:18433346.

Hart SN, Cui Y, Klaassen CD, and Zhong XB. Three patterns of cytochrome P450 gene expression during liver maturation in mice. Drug Metabolism and Disposition, 2009, 37(1):116-121. PMID:18845660. PMCID:PMC2683655.

Li Y, Cui Y, Hart SN, Klaassen CD, and Zhong XB. Dynamic histone methylation is associated with ontogenic expression of Cyp3a genes during mouse liver maturation. Molecular Pharmacology, 2009, 75(5):1171-1179. PMID:19188337, PMCID:PMC2672803.

Thomas AM, Hart SN, Kong B, Fang J, Zhong XB, and Guo GL. Genome-wide tissue-specific farnesoid X receptor binding in mouse liver and intestine. Hepatology, 2010, 51(4):1410-1419. PMID:20091679.

Hart SN, Li Y, Nakamoto K, Subileau E, Steen D, and Zhong XB. A comparison of whole genome gene expression profiles of HepaRG cells and HepG2 cells to primary human hepatocytes and human liver tissues. Drug Metabolism and Disposition, 2010, 38(6):988-994. PMID: 20228232, PMCID:PMC2879958.

Li GD, Thomas AM, Hart SN, Zhong XB, Wu D, and Guo GL. Novel insight of transcriptional activation of SHP by FXR: chromatin looping of promoter and downstream FXR response elements in Nr0b2 gene. Molecular Endocrinology, 2010, 24(7):1404-1412. PMID:20444884. PMCID:PMC2903909.

Li D, Gaedigk R, Hart SN, Leeder JS, and Zhong XB. The role of CYP3A4 mRNA transcript with shortened 3’-UTR in hepatocyte differentiation, liver development, and response to drug induction. Molecular Pharmacology, 2012, 81(1): 86-96. PMID:21998292. PMCID:PMC3250109.

Thomas AM, Hart SN, Li G, Lu H, Fang YP, Fang JW, Zhong XB, and Guo GL. Hepatocyte nuclear factor 4 alpha and farnesoid X receptor co-regulates gene transcription in mouse livers on a genome-wide scale. Pharmaceutical Research, 2013, 30(9):2188-2198. PMID:23462932. PMCID:PMC3711942.

Oral Presentations

“Single nucleotide polymorphisms in cytochrome P450 oxidoreductase influence drug metabolism” in Experimental Biology 2008 in April 5-9 in San Diego.

“Dynamic DNA and histone methylation influences the ontogeny of xenobiotic metabolism genes during mouse liver maturation” in 2009 Annual Symposium of Kansas IDeA Network of Biomedical Research Excellence in Manhattan, Kansas in January 17-18, 2009.

Poster Presentations

Li Y, Buckley D, Hart SN, Wesselman C, Nakamoto K, Klaassen CD, and Zhong XB. Genetic polymorphisms influence CYP2C9 enzyme activity for warfarin metabolism. International Heart Forum Beijing 2007, sponsored by Chinese Society of Cardiology, Beijing, China, September 21, 2007.

Hart SN, Wang S, Nakamoto K, Wesselman C, Li Y, and Zhong XB. Genetic polymorphisms in cytochrome P450 oxidoreductase influence P450-catalyzed drug metabolism and detoxification. The Annual Meeting of the Central States Chapter of the Society of Toxicology, Iowa City, September 20, 2007

Li Y, Buckley D, Wang S, Hart SN, Nakamoto K, Klaassen, CD, and Zhong XB. Genetic polymorphisms in the RNA polymerase II core promoter and enhancer elements of the UGT1A1 promoter influence its gene transcription. Experimental Biology 2008, April 5-9, San Diego.

Hart SN, Wang S, Nakamoto K, Wesselman C, Li Y, and Zhong XB. Single nucleotide polymorphisms in cytochrome P450 oxidoreductase influence drug metabolism. Experimental Biology 2008, April 5-9, San Diego.

Li Y, Cui Y, Hart SN, Klaassen CD, and Zhong XB. Dynamic histone methylation is associated with ontogenic expression of Cyp3a genes during mouse liver maturation. 2008 Annual Meeting of Central States Chapter of the Society of Toxicology, Kansas City, Kansas, September 24-25, 2008

Li Y, Cui Y, Hart SN, Klaassen CD, and Zhong XB. Dynamic histone methylation is associated with ontogenic expression of Cyp3a genes during mouse liver maturation. Keystone Symposia on Epigenetics, Development, and Human Disease, Breckenridge, Colorado, January 5-10, 2009.

Hart SN, Li Y, Cui Y, Klaassen CD, and Zhong XB. Dynamic DNA and histone methylation influences the ontogeny of xenobiotic metabolism genes during mouse liver maturation. 2009 Annual Symposium of Kansas IDeA Network of Biomedical Research Excellence, Manhattan, Kansas, January 17-18, 2009.

Li Y, Cui Y, Hart SN, Klaassen CD, and Zhong XB. Dynamic histone methylation is associated with ontogenic expression of Cyp3a genes during mouse liver maturation. 2009 Experimental Biology, New Orleans, Louisiana, April 18-22, 2009.

Hart SN, Li Y, Cui Y, Klaassen CD, and Zhong XB. Dynamic DNA and histone methylation influences the ontogeny of xenobiotic metabolism genes during mouse liver maturation. 2009 Experimental Biology, New Orleans, Louisiana, April 18-22, 2009.

Zhong XB, Li Y, Hart SN, and Nakamoto K. HepaRG cells as a model of human liver transcriptome. 16^th North American Regional Meeting of ISSX, Baltimore, Maryland, October 18-22, 2009.

Zhong XB, Li Y, Hart SN, and Nakamoto K. Epigenetic regulation of expression of the Cyp2d genes during mouse liver development. 16^th North American Regional Meeting of ISSX, Baltimore, Maryland, October 18-22, 2009.

Thomas AM, Hart SN, Zhong XB, Kong B, Fang JW, and Guo GL. Tissue-specific FXR-DNA binding: genome-wide liver and intestine binding analysis by ChIP-Seq. Hepatology, 50(4):629A. 60^th Annual Meeting of the American Association for the Study of Liver Diseases, Boston, MA, October 30-November 3, 2009.

Li D, Hart SN, Peng L, Tregear H, and Zhong XB. Surveying of alternative transcripts and splicing variants of P450 genes in response to xenobiotics by RNA sequencing. Gordon Research Conference on Drug Metabolism, Heldnerness, NH, July 11-16, 2010.

Li D, Hart SN, Peng L, Tregear H, and Zhong XB. RNA-seq reveals alternative transcripts and splicing variants of P450 genes in human hepatoma HepaRG cells in response to xenobiotics. 9^th International ISSX Meeting, Istanbul, Turkey, September 4-8, 2010.

Li GD, Thomas AM, Hart SN, Zhong XB, Wu D, and Guo GL. 2010. Farnesoid X receptor activation mediates head-to-tail chromatin looping in the Nr0b2 gene encoding small heterodimer partner. 61^th Annual Meeting of the American Association for the Study of Liver Diseases, Boston, MA, October 29-November 2, 2010.

Peng L, Hart SN, and Zhong XB. 2011. Stable transfection of CYP2D6 gene into HepaRG cells. 17^th North American Regional ISSX Meeting, Atlanta, GA, October 16-20, 2011.